|
KMID : 0613820030130050668
|
|
Journal of Life Science
2003 Volume.13 No. 5 p.668 ~ p.674
|
|
|
ACE Inhibitory Materials from Raja kenojei
|
|
Lim Hyun-Soo
|
|
|
Abstract
|
|
|
|
This study was carried out to investigate the ACE inhibitory materials of Raja kenojei. Raja kenojei was seperated to fillet and viscera, and these were extracted with hot water. Antihypertensive activity was examined by mesearing angiotensin converting enzyme ACE inhibitory activity. ACE inhibitory activity of viscera at the concentration of 2% for Day 0 showed the highest value by 71.0%. But ACE inhibitory activity of fillet at 2% showed by 29%, which was lower antihypertensive activity than viscera. The protein content of viscerial hot water extracts in proximate composition showed the highest. And also, there was a large amount of aromatic and branched aliphatic amino acids in viscera than those in fillet. For the purification of antihypertensive material in visceral hot water extracts, it was separated and collected by Sephadex G-25 gel chromatography. The fraction (B) of 111 to 160 showed the highest ACE inhibitory activity by 65.1% at the concentration of 0.05%. But the other fractions (A and C) showed lower activity than B. These results demonstrate that crude hot water extracts of viscera from Raja kenojei may be useful as functional food ingredient with antihypertensive property.
|
|
|
KEYWORD
|
|
|
Raja kenojei, viscera, fillet, ACE inhibitory activity
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|